Method And Device For Analyzing Protein-protein Interaction On Single-molecule Level In Cell Environment

Abstract

The method and apparatus that the invention discloses a kind of to analyze protein-protein interaction on single molecules level.The present invention is realized by the method for following steps: providing the first protein in the first substrate, over the substrate, biological molecular sieve is combined with the first label in the first protein, to induce the first combination marked on biological molecular sieve and the first protein；After biological molecular sieve is combined with the first label, the cytosol of the cell containing second the second albumen of label is provided in the substrate；While cytosol is supplied to the substrate, the interaction between the first protein and the second albumen is analyzed.By means of the present invention, or even the interaction between protein-protein can be being analyzed in the actual environment of cell on single molecules level.