Flap endonuclease-initiated enzymatic repairing amplification for ultrasensitive detection of target nucleic acids

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dc.contributor.authorJang, Hyowonko
dc.contributor.authorLee, Chang Yeolko
dc.contributor.authorLee, Seoyoungko
dc.contributor.authorPark, Ki Sooko
dc.contributor.authorPark, Hyun Gyuko
dc.date.accessioned2019-07-03T07:30:05Z-
dc.date.available2019-07-03T07:30:05Z-
dc.date.created2019-03-11-
dc.date.created2019-03-11-
dc.date.issued2019-02-
dc.identifier.citationNANOSCALE, v.11, no.8, pp.3633 - 3638-
dc.identifier.issn2040-3364-
dc.identifier.urihttp://hdl.handle.net/10203/262917-
dc.description.abstractA new isothermal nucleic acid amplification method termed FERA (Flap endonuclease-initiated Enzymatic Repairing Amplification) is developed for the ultrasensitive detection of target nucleic acids. In the FERA method, flap endonuclease (FEN) catalyzesthe hydrolytic cleavage at the junction of single-and double-stranded DNAs which is formed only in the presence of target nucleic acids, and releases short oligonucleotides to promote the cyclic enzymatic repairing amplification (ERA) combined with FEN-based amplification. As a result, a large amount of single-and double-stranded DNAs are generated under the isothermal conditions, leading to the high fluorescence intensity from the SYBR I green dye. Relying on the powerful amplification method, we successfully determined the target nucleic acids with a limit of detection as low as 15.16 aM, which corresponds to approximately 180 molecules in 20 mu L reaction volume, and verified the practical applicability by detecting long target nucleic acids derived from Chlamydia trachomatis.-
dc.languageEnglish-
dc.publisherROYAL SOC CHEMISTRY-
dc.titleFlap endonuclease-initiated enzymatic repairing amplification for ultrasensitive detection of target nucleic acids-
dc.typeArticle-
dc.identifier.wosid000459504400019-
dc.identifier.scopusid2-s2.0-85061974788-
dc.type.rimsART-
dc.citation.volume11-
dc.citation.issue8-
dc.citation.beginningpage3633-
dc.citation.endingpage3638-
dc.citation.publicationnameNANOSCALE-
dc.identifier.doi10.1039/c8nr06699j-
dc.contributor.localauthorPark, Hyun Gyu-
dc.contributor.nonIdAuthorPark, Ki Soo-
dc.description.isOpenAccessN-
dc.type.journalArticleArticle-
dc.subject.keywordPlusSTRAND DISPLACEMENT AMPLIFICATION-
dc.subject.keywordPlusSEQUENCE REPLICATION 3SR-
dc.subject.keywordPlusISOTHERMAL AMPLIFICATION-
dc.subject.keywordPlusSENSITIVE DETECTION-
dc.subject.keywordPlusCIRCULATING DNA-
dc.subject.keywordPlusLABEL-FREE-
dc.subject.keywordPlusPOLYMERASE-
dc.subject.keywordPlusMICRORNA-
dc.subject.keywordPlusSTRATEGY-
dc.subject.keywordPlusNASBA-
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