DC Field | Value | Language |
---|---|---|
dc.contributor.author | Sung, Pil Soo | ko |
dc.contributor.author | Park, Dong Jun | ko |
dc.contributor.author | Kim, Jung-Hee | ko |
dc.contributor.author | Han, Ji Won | ko |
dc.contributor.author | Lee, Eun Byul | ko |
dc.contributor.author | Lee, Gil Won | ko |
dc.contributor.author | Nam, Hee Chul | ko |
dc.contributor.author | Jang, Jeong Won | ko |
dc.contributor.author | Bae, Si Hyun | ko |
dc.contributor.author | Choi, Jong Young | ko |
dc.contributor.author | Shin, Eui-Cheol | ko |
dc.contributor.author | Park, Su-Hyung | ko |
dc.contributor.author | Yoon, Seung Kew | ko |
dc.date.accessioned | 2019-06-24T03:10:11Z | - |
dc.date.available | 2019-06-24T03:10:11Z | - |
dc.date.created | 2019-06-24 | - |
dc.date.created | 2019-06-24 | - |
dc.date.issued | 2019-06 | - |
dc.identifier.citation | FRONTIERS IN IMMUNOLOGY, v.10 | - |
dc.identifier.issn | 1664-3224 | - |
dc.identifier.uri | http://hdl.handle.net/10203/262804 | - |
dc.description.abstract | In this study, we aimed to detect and characterize ex vivo virus-specific CD8(+) T cells in patients with immune-tolerant hepatitis B virus (HBV) infection. We investigated a Korean chronic hepatitis B cohort composed of 15 patients in the immune-tolerant phase, 17 in the immune-active phase, and 13 under antiviral treatment. We performed enzyme-linked immunospot (ELISpot) assays ex vivo and intracellular cytokine staining after in vitro culture. We also performed ex vivo multimer staining assays and examined the expression of programmed death-1 (PD-1) and CD127 in pentamer-positive cells. Ex vivo ELISpot revealed that HBV-specific T cell function was weaker in immune-tolerant patients than in those under antiviral treatment. In vitro culture of peripheral blood mononuclear cells for 10 days revealed that HBV-specific CD8(+) T cells produced interferon-y in some immune-tolerant patients. We detected HBV-specific CD8(+) T cells ex vivo (using the HBV core(18-27) pentamer) in patients from all three groups. The PD-1(+) subset of pentamer(+) CD8(+) T cells was smaller ex vivo in the immune-tolerant phase than in the immune-active phase or under antiviral treatment. Interestingly, the proportion of PD-1(+) CD8(+) T cells in HBV-specific CD8(+) T cells correlated with patient age when all enrolled patients were analyzed. Overall, HBV-specific CD8(+) T cells are present in patients considered as immune-tolerant, although their ex vivo functionality is significantly weaker than that in patients under antiviral treatment (P < 0.05). Despite the high viral load, the proportion of PD-1 expression in HBV-specific CD8(+) T cells is lower in the immune-tolerant phase than in other phases. Our results indicate appropriate stimulation may enhance the effector function of HBV-specific CD8(+) T cells in patients considered as being in the immune-tolerant phase. | - |
dc.language | English | - |
dc.publisher | FRONTIERS MEDIA SA | - |
dc.title | Ex vivo Detection and Characterization of Hepatitis B Virus-Specific CD8(+) T Cells in Patients Considered Immune Tolerant | - |
dc.type | Article | - |
dc.identifier.wosid | 000470988100001 | - |
dc.identifier.scopusid | 2-s2.0-85068931569 | - |
dc.type.rims | ART | - |
dc.citation.volume | 10 | - |
dc.citation.publicationname | FRONTIERS IN IMMUNOLOGY | - |
dc.identifier.doi | 10.3389/fimmu.2019.01319 | - |
dc.contributor.localauthor | Shin, Eui-Cheol | - |
dc.contributor.localauthor | Park, Su-Hyung | - |
dc.contributor.nonIdAuthor | Park, Dong Jun | - |
dc.contributor.nonIdAuthor | Kim, Jung-Hee | - |
dc.contributor.nonIdAuthor | Lee, Eun Byul | - |
dc.contributor.nonIdAuthor | Lee, Gil Won | - |
dc.contributor.nonIdAuthor | Nam, Hee Chul | - |
dc.contributor.nonIdAuthor | Jang, Jeong Won | - |
dc.contributor.nonIdAuthor | Bae, Si Hyun | - |
dc.contributor.nonIdAuthor | Choi, Jong Young | - |
dc.contributor.nonIdAuthor | Yoon, Seung Kew | - |
dc.description.isOpenAccess | Y | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordAuthor | hepatitis B virus | - |
dc.subject.keywordAuthor | CD8(+) T-cell response | - |
dc.subject.keywordAuthor | programmed death protein-1 | - |
dc.subject.keywordAuthor | chronic infection | - |
dc.subject.keywordAuthor | interferon-gamma | - |
dc.subject.keywordPlus | GENOTYPE-C | - |
dc.subject.keywordPlus | HIGH PREVALENCE | - |
dc.subject.keywordPlus | UP-REGULATION | - |
dc.subject.keywordPlus | INFECTION | - |
dc.subject.keywordPlus | RESPONSES | - |
dc.subject.keywordPlus | GUIDELINES | - |
dc.subject.keywordPlus | DYSFUNCTION | - |
dc.subject.keywordPlus | EXPRESSION | - |
dc.subject.keywordPlus | MANAGEMENT | - |
dc.subject.keywordPlus | KINETICS | - |
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