Solar-Driven Biocatalytic C-Hydroxylation through Direct Transfer of Photoinduced Electrons

Cited 0 time in webofscience Cited 0 time in scopus
  • Hit : 84
  • Download : 0
Despite the immense potential of P450s, the dependence on the nicotinamide cofactor (NADPH) and NADPH-P450 reductase (CPR) limits their employment in the chemical industry. Here, we present a visible light-driven platform for biocatalytic C-hydroxylation reactions using natural flavin molecules, especially flavin mononucleotide, as a photosensitizer. By employing visible light as a source of energy instead of the nicotinamide cofactor, the bacterial CYP102A1 heme domain was successfully applied for photobiocatalytic C-hydroxylation of 4-nitrophenol and lauric acid – in the absence of NADPH and CPR. We present a proof of concept that the photoactivation of flavins is productively coupled with the direct transfer of photoinduced electrons to the P450 heme iron, achieving photobiocatalytic C-hydroxylation reactions.
Publisher
ROYAL SOC CHEMISTRY
Issue Date
2019-02
Language
English
Article Type
Article
Citation

GREEN CHEMISTRY, v.21, no.3, pp.515 - 525

ISSN
1463-9262
DOI
10.1039/C8GC02398K
URI
http://hdl.handle.net/10203/251643
Appears in Collection
CBE-Journal Papers(저널논문)MS-Journal Papers(저널논문)
Files in This Item
There are no files associated with this item.

qr_code

  • mendeley

    citeulike


rss_1.0 rss_2.0 atom_1.0