DC Field | Value | Language |
---|---|---|
dc.contributor.author | Ryu, Yiseul | ko |
dc.contributor.author | Kang, Jung Ae | ko |
dc.contributor.author | Kim, Dasom | ko |
dc.contributor.author | Kim, Song-Rae | ko |
dc.contributor.author | Kim, Seungmin | ko |
dc.contributor.author | Park, Seong Ji | ko |
dc.contributor.author | Kwon, Seung-Hae | ko |
dc.contributor.author | Kim, Kil-Nam | ko |
dc.contributor.author | Lee, Dong-Eun | ko |
dc.contributor.author | Lee, Joong-jae | ko |
dc.contributor.author | Kim, Hak-Sung | ko |
dc.date.accessioned | 2019-02-20T05:15:35Z | - |
dc.date.available | 2019-02-20T05:15:35Z | - |
dc.date.created | 2019-02-07 | - |
dc.date.issued | 2018-12 | - |
dc.identifier.citation | SMALL, v.14, no.52 | - |
dc.identifier.issn | 1613-6810 | - |
dc.identifier.uri | http://hdl.handle.net/10203/250417 | - |
dc.description.abstract | With a growing number of intracellular drug targets and the high efficacy of protein therapeutics, the targeted delivery of active proteins with negligible toxicity is a challenging issue in the field of precision medicine. Herein, a programed assembly of nucleoprotein nanoparticles (NNPs) using DNA and zinc fingers (ZnFs) for targeted protein delivery is presented. Two types of ZnFs with different sequence specificities are genetically fused to a targeting moiety and a protein cargo, respectively. Double-stranded DNA with multiple ZnF-binding sequences is grafted onto inorganic nanoparticles, followed by conjugation with the ZnF-fused proteins, generating the assembly of NNPs with a uniform size distribution and high stability. The approach enables controlled loading of a protein cargo on the NNPs, offering a high cytosolic delivery efficiency and target specificity. The utility and potential of the assembly as a versatile protein delivery vehicle is demonstrated based on their remarkable antitumor activity and target specificity with negligible toxicity in a xenograft mice model. | - |
dc.language | English | - |
dc.publisher | WILEY-V C H VERLAG GMBH | - |
dc.title | Programed Assembly of Nucleoprotein Nanoparticles Using DNA and Zinc Fingers for Targeted Protein Delivery | - |
dc.type | Article | - |
dc.identifier.wosid | 000456505700001 | - |
dc.identifier.scopusid | 2-s2.0-85056146008 | - |
dc.type.rims | ART | - |
dc.citation.volume | 14 | - |
dc.citation.issue | 52 | - |
dc.citation.publicationname | SMALL | - |
dc.identifier.doi | 10.1002/smll.201802618 | - |
dc.contributor.localauthor | Kim, Hak-Sung | - |
dc.contributor.nonIdAuthor | Kang, Jung Ae | - |
dc.contributor.nonIdAuthor | Kim, Song-Rae | - |
dc.contributor.nonIdAuthor | Kim, Seungmin | - |
dc.contributor.nonIdAuthor | Park, Seong Ji | - |
dc.contributor.nonIdAuthor | Kwon, Seung-Hae | - |
dc.contributor.nonIdAuthor | Kim, Kil-Nam | - |
dc.contributor.nonIdAuthor | Lee, Dong-Eun | - |
dc.contributor.nonIdAuthor | Lee, Joong-jae | - |
dc.description.isOpenAccess | N | - |
dc.type.journalArticle | Article | - |
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