Programed Assembly of Nucleoprotein Nanoparticles Using DNA and Zinc Fingers for Targeted Protein Delivery

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dc.contributor.authorRyu, Yiseulko
dc.contributor.authorKang, Jung Aeko
dc.contributor.authorKim, Dasomko
dc.contributor.authorKim, Song-Raeko
dc.contributor.authorKim, Seungminko
dc.contributor.authorPark, Seong Jiko
dc.contributor.authorKwon, Seung-Haeko
dc.contributor.authorKim, Kil-Namko
dc.contributor.authorLee, Dong-Eunko
dc.contributor.authorLee, Joong-jaeko
dc.contributor.authorKim, Hak-Sungko
dc.date.accessioned2019-02-20T05:15:35Z-
dc.date.available2019-02-20T05:15:35Z-
dc.date.created2019-02-07-
dc.date.issued2018-12-
dc.identifier.citationSMALL, v.14, no.52-
dc.identifier.issn1613-6810-
dc.identifier.urihttp://hdl.handle.net/10203/250417-
dc.description.abstractWith a growing number of intracellular drug targets and the high efficacy of protein therapeutics, the targeted delivery of active proteins with negligible toxicity is a challenging issue in the field of precision medicine. Herein, a programed assembly of nucleoprotein nanoparticles (NNPs) using DNA and zinc fingers (ZnFs) for targeted protein delivery is presented. Two types of ZnFs with different sequence specificities are genetically fused to a targeting moiety and a protein cargo, respectively. Double-stranded DNA with multiple ZnF-binding sequences is grafted onto inorganic nanoparticles, followed by conjugation with the ZnF-fused proteins, generating the assembly of NNPs with a uniform size distribution and high stability. The approach enables controlled loading of a protein cargo on the NNPs, offering a high cytosolic delivery efficiency and target specificity. The utility and potential of the assembly as a versatile protein delivery vehicle is demonstrated based on their remarkable antitumor activity and target specificity with negligible toxicity in a xenograft mice model.-
dc.languageEnglish-
dc.publisherWILEY-V C H VERLAG GMBH-
dc.titleProgramed Assembly of Nucleoprotein Nanoparticles Using DNA and Zinc Fingers for Targeted Protein Delivery-
dc.typeArticle-
dc.identifier.wosid000456505700001-
dc.identifier.scopusid2-s2.0-85056146008-
dc.type.rimsART-
dc.citation.volume14-
dc.citation.issue52-
dc.citation.publicationnameSMALL-
dc.identifier.doi10.1002/smll.201802618-
dc.contributor.localauthorKim, Hak-Sung-
dc.contributor.nonIdAuthorKang, Jung Ae-
dc.contributor.nonIdAuthorKim, Song-Rae-
dc.contributor.nonIdAuthorKim, Seungmin-
dc.contributor.nonIdAuthorPark, Seong Ji-
dc.contributor.nonIdAuthorKwon, Seung-Hae-
dc.contributor.nonIdAuthorKim, Kil-Nam-
dc.contributor.nonIdAuthorLee, Dong-Eun-
dc.contributor.nonIdAuthorLee, Joong-jae-
dc.description.isOpenAccessN-
dc.type.journalArticleArticle-
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