Rapid and label-free, electrochemical DNA detection utilizing the oxidase-mimicking activity of cerium oxide nanoparticles

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We herein developed a simple and ultrafast electrochemical DNA detection method by utilizing the oxidase-mimicking activity of cerium oxide nanoparticles (CeO2). In the sensor, the presence of DNA, which electrostatically interacts with CeO2 and 3,3', 5,5'-tetramethylbenzidine (TMB) substrate, limits the formation of CeO2-TMB complex that is required for the oxidase-like reaction. As a result, the oxidase-mimicking activity of CeO2 is not effectively exerted, leading to the significantly diminished electrochemical current signal. With this novel strategy, we successfully determined DNA amplicon derived from Escherichia coli (E. coli) with the total assay time less than 6 min even without the post-purification of DNA amplicon.
Publisher
ELSEVIER SCIENCE INC
Issue Date
2019-02
Language
English
Article Type
Article
Citation

ELECTROCHEMISTRY COMMUNICATIONS, v.99, pp.5 - 10

ISSN
1388-2481
DOI
10.1016/j.elecom.2018.12.008
URI
http://hdl.handle.net/10203/250350
Appears in Collection
CBE-Journal Papers(저널논문)
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