DC Field | Value | Language |
---|---|---|
dc.contributor.author | Jung, Yieun | ko |
dc.contributor.author | Ahn, So-Hee | ko |
dc.contributor.author | Park, Hyunju | ko |
dc.contributor.author | Park, Sang Hui | ko |
dc.contributor.author | Choi, Kyungsun | ko |
dc.contributor.author | Choi, Chulhee | ko |
dc.contributor.author | Kang, Jihee Lee | ko |
dc.contributor.author | Choi, Youn-Hee | ko |
dc.date.accessioned | 2018-10-19T00:29:50Z | - |
dc.date.available | 2018-10-19T00:29:50Z | - |
dc.date.created | 2018-09-27 | - |
dc.date.created | 2018-09-27 | - |
dc.date.issued | 2018-09 | - |
dc.identifier.citation | CELLULAR PHYSIOLOGY AND BIOCHEMISTRY, v.48, no.3, pp.1332 - 1346 | - |
dc.identifier.issn | 1015-8987 | - |
dc.identifier.uri | http://hdl.handle.net/10203/245885 | - |
dc.description.abstract | Background/Aims: Glioblastoma multiforme (GBM) is the most common primary brain tumor in adults. The defining characteristics of GBM are diffuse infiltration of tumor cells into normal brain parenchyma, rapid growth, a high degree of infiltration of microglia and macrophages, and the presence of necrosis. Microglia/macrophages are frequently found in gliomas and they extensively infiltrate GBM tissue, up to 30% of total tumor mass. However, little is known about the effect of necrotic cells (NCs) on microglia infiltration in GBM and the tumor-infiltrating microglia-induced factors in GBMs. Methods: In this study, to address whether necrosis or necrosis-exposed GBM cells affect the degree of microglia/macrophage infiltration, migration and invasion/infiltration assays were performed. Culture supernatants and nuclear extracts of CRT-MG cells treated or untreated with necrotic cells were analyzed using a chemokine array and electrophoretic mobility shift assay, respectively. Results: The presence of NCs promoted the migration/infiltration of microglia, and GBM cell line CRT-MG cells exposed to NCs further enhanced the migration and infiltration of HM06 microglial cells. Treatment with NCs induced mRNA and protein expression of chemokines such as Monocyte Chemoattractant Protein-1 (CCL2/MCP-1) and Macrophage Inflammatory Protein-3 alpha (CCL20/MIP-3 alpha) in CRT MG cells. In particular, CCL2/MCP-1 and CCL20/MIP-3 alpha were significantly increased in NC-treated CRT-MG cells. NCs induced DNA binding of the transcription factors Nuclear Factor (NF)-kappa B and Activator Protein 1 (AP-1) to the CCL2/MCP-1 and CCL20/MIP-3 alpha promoters, leading to increased CCL2/MCP-1 and CCL20/MIP-3 alpha mRNA and protein expression in CRT MG cells. Conclusion: These results provide evidence that NCs induce the expression of CCL2/MCP-1 and CCL20/MIP-3 alpha in glioblastoma cells through activation of NF-kappa B and AP-1 and facilitate the infiltration of microglia into tumor tissues. (C) 2018 The Author(s) Published by S Karger AG, Basel | - |
dc.language | English | - |
dc.publisher | KARGER | - |
dc.title | MCP-1 and MIP-3 alpha Secreted from Necrotic Cell-Treated Glioblastoma Cells Promote Migration/Infiltration of Microglia | - |
dc.type | Article | - |
dc.identifier.wosid | 000443612500037 | - |
dc.identifier.scopusid | 2-s2.0-85052486649 | - |
dc.type.rims | ART | - |
dc.citation.volume | 48 | - |
dc.citation.issue | 3 | - |
dc.citation.beginningpage | 1332 | - |
dc.citation.endingpage | 1346 | - |
dc.citation.publicationname | CELLULAR PHYSIOLOGY AND BIOCHEMISTRY | - |
dc.identifier.doi | 10.1159/000492092 | - |
dc.contributor.localauthor | Choi, Chulhee | - |
dc.contributor.nonIdAuthor | Jung, Yieun | - |
dc.contributor.nonIdAuthor | Ahn, So-Hee | - |
dc.contributor.nonIdAuthor | Park, Hyunju | - |
dc.contributor.nonIdAuthor | Park, Sang Hui | - |
dc.contributor.nonIdAuthor | Choi, Kyungsun | - |
dc.contributor.nonIdAuthor | Kang, Jihee Lee | - |
dc.contributor.nonIdAuthor | Choi, Youn-Hee | - |
dc.description.isOpenAccess | N | - |
dc.type.journalArticle | Article | - |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.