Repeat Module-Based Rational Design of a Photoswitchable Protein for Light-Driven Control of Biological Processes

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Light-driven control of biological processes using photoswitchable proteins allows high spatiotemporal interrogation or manipulation of such processes, assisting in understanding their functions. Despite considerable advances, however, the wide spread use of optical control has been hampered by a limited repertoire of photoswitchable proteins and a lack of generalized design strategy. Herein, we present a repeat module-based rational design of a photoswitchable protein composed of LRR (Leucine-rich repeat) modules using azobenzene as a photochromic ligand. Our design approach involves the rational selection of a C-beta pair between two nearby modules within a convex region and subsequent cross-linking with a photochromic ligand. We demonstrate the general utility and potential of our strategy by showing the design of three target-specific photoswitchable proteins and a light-driven modulation of the cell signaling. With an abundance of LRR proteins in nature, our approach can expand the repertoire of photoswitchable proteins for light-driven control of biological processes.
Publisher
WILEY-V C H VERLAG GMBH
Issue Date
2018-08
Language
English
Article Type
Article
Keywords

LIVING CELLS; AZOBENZENE; PEPTIDES; BINDER

Citation

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, v.57, no.34, pp.10859 - 10863

ISSN
1433-7851
DOI
10.1002/anie.201803993
URI
http://hdl.handle.net/10203/245675
Appears in Collection
BS-Journal Papers(저널논문)
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