Nanoscale imaging of RNA with expansion microscopy

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The ability to image RNA identity and location with nanoscale precision in intact tissues is of great interest for defining cell types and states in normal and pathological biological settings. Here, we present a strategy for expansion microscopy of RNA. We developed a small-molecule linker that enables RNA to be covalently attached to a swellable polyelectrolyte gel synthesized throughout a biological specimen. Then, postexpansion, fluorescent in situ hybridization (FISH) imaging of RNA can be performed with high yield and specificity as well as single-molecule precision in both cultured cells and intact brain tissue. Expansion FISH (ExFISH) separates RNAs and supports amplification of single-molecule signals (i.e., via hybridization chain reaction) as well as multiplexed RNA FISH readout. ExFISH thus enables super-resolution imaging of RNA structure and location with diffraction-limited microscopes in thick specimens, such as intact brain tissue and other tissues of importance to biology and medicine.
Publisher
NATURE PUBLISHING GROUP
Issue Date
2016-08
Language
English
Article Type
Article
Keywords

X-CHROMOSOME INACTIVATION; SINGLY LABELED PROBES; IN-SITU HYBRIDIZATION; MESSENGER-RNA; FLUORESCENT PROTEINS; XIST RNA; TRANSCRIPTS; PLATFORM; CELL; LOCALIZATION

Citation

NATURE METHODS, v.13, no.8, pp.679 - +

ISSN
1548-7091
DOI
10.1038/nmeth.3899
URI
http://hdl.handle.net/10203/245589
Appears in Collection
MS-Journal Papers(저널논문)
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