Electrophoresis assisted time-of-flow mass spectrometry using hollow nanomechanical resonators

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This report discusses the first demonstration of electrophoresis assisted time-of-flow mass spectrometry using 'U' shaped hollow nanomechanical resonators (HNR). Capillary electrophoresis was coupled with the HNR based mass detection to overcome low ionic conductivity of channels embedded in the HNR preventing direct in-situ electrophoretic separation. The flow of analytes through the HNR was achieved by balancing the hydrodynamic pressure to override the electromotive force and inhibit the motion of analytes towards the anode for capillary electrophoresis. The resonance frequency shifts of the HNR vibrating around 1.5 MHz were correlated with the time of the passage of the protein bands to construct the mass spectrum. The proposed concept was demonstrated by constructing a mass spectrum of egg white proteins in the molecular weight range of 14-250 kDa. When compared to regular polyacrylamide gel electrophoresis, our method not only provides a precise and fast readout but also avoids the use of chemical staining. This study paves a new route for low-cost and on-chip mass spectrometers with ultra-miniaturized dimensions.
Publisher
NATURE PUBLISHING GROUP
Issue Date
2017-06
Language
English
Article Type
Article
Keywords

SUSPENDED MICROCHANNEL RESONATORS; ION CONCENTRATION POLARIZATION; SINGLE CELLS; RESOLUTION; DENSITY; CARBON; SENSOR; VOLUME; FLUID

Citation

SCIENTIFIC REPORTS, v.7

ISSN
2045-2322
DOI
10.1038/s41598-017-03846-y
URI
http://hdl.handle.net/10203/245445
Appears in Collection
ME-Journal Papers(저널논문)
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