Electrostatically assembled dendrimer complex with a high-affinity protein binder for targeted gene delivery

Cited 3 time in webofscience Cited 0 time in scopus
  • Hit : 79
  • Download : 0
Although a variety of non-viral gene delivery systems have been developed, they still suffer from low efficiency and specificity. Herein, we present the assembly of a dendrimer complex comprising a DNA cargo and a targeting moiety as a new format for targeted gene delivery. A PAMAM dendrimer modified with histidine and arginine (HR-dendrimer) was used to enhance the endosomal escape and transfection efficiency. An EGFR-specific repebody, composed of leucine-rich repeat (LRR) modules, was employed as a targeting moiety. A polyanionic peptide was genetically fused to the repebody, followed by incubation with an HR-dendrimer and a DNA cargo to assemble the dendrimer complex through an electrostatic interaction. The resulting dendrimer complex was shown to deliver a DNA cargo with high efficiency in a receptor-specific manner. An analysis using a confocal microscope confirmed the internalization of the dendrimer complex and subsequent dissociation of a DNA cargo from the complex. The present approach can be broadly used in a targeted gene delivery in many areas.
Publisher
ELSEVIER SCIENCE BV
Issue Date
2018-06
Language
English
Article Type
Article
Keywords

DRUG-DELIVERY; CANCER-THERAPY; NANOPARTICLES; POLYMERS; DESIGN; CELLS; TUMOR; DERIVATIVES; EXPRESSION; CARRIER

Citation

INTERNATIONAL JOURNAL OF PHARMACEUTICS, v.544, no.1, pp.39 - 45

ISSN
0378-5173
DOI
10.1016/j.ijpharm.2018.04.015
URI
http://hdl.handle.net/10203/243691
Appears in Collection
BS-Journal Papers(저널논문)
Files in This Item
There are no files associated with this item.
This item is cited by other documents in WoS
⊙ Detail Information in WoSⓡ Click to see webofscience_button
⊙ Cited 3 items in WoS Click to see citing articles in records_button

qr_code

  • mendeley

    citeulike


rss_1.0 rss_2.0 atom_1.0