Investigation of the characteristic of G-quadruplex in high viscosity condition높은 점성도 하의 G-quadruplex의 성질 연구

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Guanine (G)-quadruplex is of interest due to its appearance in the telomere sequence, the oncogene promoter region, etc. Its diffusion and change of structure, especially under high viscosity, are important for understanding its dynamics. In this paper, home-built Fluorescence Correlation Spectroscopy (FCS) is introduced to obtain the diffusion properties of DNA. The system was calibrated with Rhodamine 6G and TAMRA. Also, cross correlation system was installed to overcome the afterpulsing effect. By this FCS system, the difference in the diffusion dynamics of the G-rich DNA sequences of single-strand DNA (ssDNA) and the G-quadruplex in aqueous, sucrose, and polyethylene glycol (PEG) solutions. From experiments with aqueous and sucrose solutions, we confirm that a simple diffusion model according to the viscosity is appropriate. In the PEG experiments, the nanoviscosity effect is observed according to PEG’s molecular weight. In the PEG 200 solution, both the ssDNA and the G-quadruplex possess macroviscosity. In the PEG 10000 solution, the G-quadruplex possesses nanoviscosity and the ssDNA possesses macroviscosity, whereas, in the PEG 35000 solution, both ssDNA and the G-quadruplex possess nanoviscosity. The experimental results are consistent with the theoretical predictions that G-quadruplexes may have less effective viscosity (nanoviscosity) when they are smaller than the solvent molecules.
Advisors
Kim, Sooyongresearcher김수용researcher
Description
한국과학기술원 :물리학과,
Publisher
한국과학기술원
Issue Date
2017
Identifier
325007
Language
eng
Description

학위논문(박사) - 한국과학기술원 : 물리학과, 2017.2,[vii, 53 p. :]

Keywords

Fluorescence correlation spectroscopy; Fluorescence; G-quadruplex; nanoviscosity; molecular diffusion; 형광 상관 분광법; 형광; 분자 확산운동

URI
http://hdl.handle.net/10203/241767
Link
http://library.kaist.ac.kr/search/detail/view.do?bibCtrlNo=675688&flag=dissertation
Appears in Collection
PH-Theses_Ph.D.(박사논문)
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