Activation of NLRP3 and AIM2 Inflammasomes by Porphyromonas gingivalis Infection

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Porphyromonas gingivalis, a major periodontopathogen, is involved in the pathogenesis of periodontitis. Interleukin-1 beta (IL-1 beta), a proinflammatory cytokine, regulates innate immune responses and is critical for the host defense against bacterial infection. However, excessive IL-1 beta is linked to periodontal destruction. IL-1 beta synthesis, maturation, and secretion are tightly regulated by Toll-like receptor (TLR) signaling and inflammasome activation. We found much higher levels of inflammasome components in the gingival tissues from patients with chronic periodontitis than in those from healthy controls. To investigate the molecular mechanisms by which P. gingivalis infection causes IL-1 beta secretion, we examined the characteristics of P. gingivalis-induced signaling in differentiated THP-1 cells. We found that P. gingivalis induces IL-1 beta secretion and inflammatory cell death via caspase-1 activation. We also found that P. gingivalis-induced IL-1 beta secretion and pyroptic cell death required both NLRP3 and AIM2 inflammasome activation. The activation of the NLRP3 inflammasome was mediated by ATP release, the P2X(7) receptor, and lysosomal damage. In addition, we found that the priming signal via TLR2 and TLR4 activation precedes P. gingivalis- induced IL-1 beta release. Our study provides novel insight into the innate immune response against P. gingivalis infection which could potentially be used for the prevention and therapy of periodontitis.
Publisher
AMER SOC MICROBIOLOGY
Issue Date
2014-01
Language
English
Article Type
Article
Keywords

TOLL-LIKE RECEPTORS; CELL-DEATH; LISTERIA-MONOCYTOGENES; CASPASE-1 ACTIVATION; NALP3 INFLAMMASOME; IMMUNE-RESPONSES; INNATE IMMUNITY; HUMAN MONOCYTES; CUTTING EDGE; PROTEIN

Citation

INFECTION AND IMMUNITY, v.82, no.1, pp.112 - 123

ISSN
0019-9567
DOI
10.1128/IAI.00862-13
URI
http://hdl.handle.net/10203/240799
Appears in Collection
MSE-Journal Papers(저널논문)
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