Method for analyzing protein-protein interaction on single-molecule level in cell environment, and method for measuring density of protein activated in cytosol 세포 용액에서의 활성화된 단백질 농도 측정 방법

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A method of analyzing protein-protein interactions includes: preparing two substrates, in which first protein-binding molecules that are biomolecules to be bound to the first proteins are attached to each of the substrates; inducing binding between the first proteins and the first protein-binding molecules on the first substrate and the second substrate, respectively, by supplying the first proteins included in the control group-cell to the first substrate among the two substrates and supplying the first proteins included in the experimental group-cell to the second substrate among the two substrates; supplying cell lysates of cells including the marker-tagged second proteins to the first substrate and the second substrate, respectively; and comparing the interactions between the first proteins and the second proteins on the first substrate and the second substrate in the supply of the cell lysates to the first substrate and the second substrate, respectively.
Assignee
KAIST
Country
US (United States)
Issue Date
2016-06-28
Application Date
2013-10-21
Application Number
14059294
Registration Date
2016-06-28
Registration Number
9377462
URI
http://hdl.handle.net/10203/230614
Appears in Collection
PH-Patent(특허)
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