Method and apparatus for analyzing protein-protein interaction on single-molecule level within the cellular environment 셀룰러 환경 내에 단일 분자 레벨상에서 단백질-단백질 상호작용을 분석하기 위한 방법과 장치

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A method of analyzing protein-protein interactions includes binding the first proteins to the substrate where the first proteins are tagged with the first markers which bind specifically to the biomolecules immobilized on the substrate or the first proteins bind specifically to the biomolecules immobilized on the substrate; incubating the substrate bound first proteins with cell lysate containing the second proteins which are tagged with second markers; analyzing the interactions between the first proteins and the second proteins in the cell lysate, and obtaining the first analytic value representing the kinetic picture of the interactions; incubating the substrate bound first proteins with cell lysate mixture of a cell lysate consisting of the second markers-tagged second proteins and another cell lysate comprising other proteins including unlabelled second proteins and obtaining the second analytic value; comparing and analyzing the first and the second analytic values.
Assignee
KAIST
Country
US (United States)
Issue Date
2016-08-23
Application Date
2013-10-21
Application Number
14059349
Registration Date
2016-08-23
Registration Number
9423400
URI
http://hdl.handle.net/10203/230491
Appears in Collection
PH-Patent(특허)
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