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College of Engineering(공과대학)Dept. of Chemical and Biomolecular Engineering(생명화학공학과)CBE-Journal Papers(저널논문)

Influence of gluconeogenic phosphoenolpyruvate carboxykinase (PCK) expression on succinic acid fermentation in Escherichia coli under high bicarbonate condition

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dc.contributor.authorKwon, YDko
dc.contributor.authorLee, SangYupko
dc.contributor.authorKim, Pko
dc.date.accessioned2011-03-18T05:37:57Z-
dc.date.available2011-03-18T05:37:57Z-
dc.date.created2012-02-06-
dc.date.created2012-02-06-
dc.date.created2012-02-06-
dc.date.issued2006-09-
dc.identifier.citationJOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY, v.16, pp.1448 - 1452-
dc.identifier.issn1017-7825-
dc.identifier.urihttp://hdl.handle.net/10203/22797-
dc.description.abstractThe effects of amplifying the gluconeogenic phosphoenolpyruvate carboxykinase of Escherichia coli ((pck(Ec)) on succinic acid production in E. coli were examined under anaerobic condition. No significant increase in succinic acid production was observed in E coli overexpressing the pck(Ec) gene without supplementing NaHCO3 or MgCO3, On the other hand, succinic acid production was enhanced as the NaHCO3 concentration was increased. When 20 g/l of NaHCO3 was added, succinic acid production in recombinant E coli overexpressing PCK was 2.2-fold higher than that observed in the wild-type strain. It was concluded that the gluconeogenic pck(Ec) overexpression enabled E. coli to enhance succinic acid production only under the high bicarbonate supplementation condition.-
dc.description.sponsorshipThis work was funded by the Genome-based Integrated Bioprocess Development Project from the Korean Ministry of Science and Technology. P. Kim was partly supported by the Catholic University of Korea research fund 2006.en
dc.languageEnglish-
dc.language.isoen_USen
dc.publisherKOREAN SOC MICROBIOLOGY & BIOTECHNOLOGY-
dc.titleInfluence of gluconeogenic phosphoenolpyruvate carboxykinase (PCK) expression on succinic acid fermentation in Escherichia coli under high bicarbonate condition-
dc.typeArticle-
dc.identifier.wosid000240892200018-
dc.identifier.scopusid2-s2.0-33750196846-
dc.type.rimsART-
dc.citation.volume16-
dc.citation.beginningpage1448-
dc.citation.endingpage1452-
dc.citation.publicationnameJOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY-
dc.embargo.liftdate9999-12-31-
dc.embargo.terms9999-12-31-
dc.contributor.localauthorLee, SangYup-
dc.contributor.nonIdAuthorKwon, YD-
dc.contributor.nonIdAuthorKim, P-
dc.type.journalArticleArticle-
dc.subject.keywordAuthorEscherichia coli-
dc.subject.keywordAuthorsuccinic acid-
dc.subject.keywordAuthorphosphoenol-pyruvate carboxykinase-
dc.subject.keywordAuthorNaHCO3-
dc.subject.keywordPlusMULTIPLE EFFECTORS INVIVO-
dc.subject.keywordPlusPYRUVATE-CARBOXYLASE-
dc.subject.keywordPlusGROWTH-
dc.subject.keywordPlusOVEREXPRESSION-
dc.subject.keywordPlusGLUCOSE-
dc.subject.keywordPlusSUCCINICIPRODUCENS-
dc.subject.keywordPlusPATHWAY-
dc.subject.keywordPlusMUTANT-
dc.subject.keywordPlusRUMEN-
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