Fluorescent peptide indicator displacement assay for monitoring interactions between RNA and RNA binding proteins

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This paper describes a sensitive, non-destructive displacement assay, using a fluorescent peptide indicator, for real-time monitoring of the interactions between RNA and RNA binding proteins (RBPs). The developed fluorescent peptide indicators, each containing a mid-sequence fluorophore unit, allowed sensing of target RNA and RNA-RBP interactions through changes in fluorescence intensity. We anticipate that this assay will open up new possibilities for meaningful studies of RNA-RBP interactions.
Publisher
ROYAL SOC CHEMISTRY
Issue Date
2013
Language
English
Article Type
Article
Keywords

PHOTOINDUCED ELECTRON-TRANSFER; BACTERIOPHAGE-LAMBDA; N PROTEIN; COMPLEX; ANTITERMINATION; RECOGNITION; SELECTIVITY; AFFINITY

Citation

MOLECULAR BIOSYSTEMS, v.9, no.5, pp.948 - 951

ISSN
1742-206X
DOI
10.1039/c2mb25470k
URI
http://hdl.handle.net/10203/225381
Appears in Collection
CH-Journal Papers(저널논문)
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