Naked Eye Detection of Salmonella typhimurium Using Scanometric Antibody Probe

Cited 0 time in webofscience Cited 0 time in scopus
  • Hit : 337
  • Download : 0
Salmonella is one of the most common foodborne pathogens, and Salmonella outbreaks are mostly associated with the intake of contaminated food or drink. Therefore, the rapid and sensitive on-site detection of Salmonella is very important. We report a naked eye detection method for Salmonella typhimurium using scanometric antibody probe. The antibody-attached glass substrate was treated with Salmonella typhimurium and the scanometric antibody probe was applied. After Ag enhancement of the probe, Salmonella typhimurium could be detected with the naked eye. The scanometric antibody probe was prepared by simply mixing Au nanoparticles, gold binding peptide-protein G, and antibody against Salmonella typhimurium. This probe can act as a signal enhancer and thus allows for an extremely simple, rapid, and efficient analysis of Salmonella typhimurium by the naked eye. We detected Salmonella typhimurium at a low concentration of 10(3) CFU/ml and clearly distinguished this bacterium from other foodborne pathogens. Furthermore, we successfully detected Salmonella typhimurium in milk, suggesting that this method can be useful in real-life samples. Because the scanometric antibody probe can be expanded to various types of antibodies, this naked eye detection method could be employed for the detection of various types of pathogens.
Publisher
AMER SCIENTIFIC PUBLISHERS
Issue Date
2017-07
Language
English
Article Type
Article
Keywords

FOODBORNE ILLNESS; UNITED-STATES; FOOD SAFETY; PROTEIN-G; SURFACE; NANOPARTICLES; IMMUNOASSAY; ENVIRONMENT; PATHOGENS; BINDING

Citation

JOURNAL OF NANOSCIENCE AND NANOTECHNOLOGY, v.17, no.7, pp.4608 - 4612

ISSN
1533-4880
DOI
10.1166/jnn.2017.14238
URI
http://hdl.handle.net/10203/224517
Appears in Collection
CH-Journal Papers(저널논문)
Files in This Item
There are no files associated with this item.

qr_code

  • mendeley

    citeulike


rss_1.0 rss_2.0 atom_1.0