A bioorthogonal approach for imaging the binding between Dasatinib and its target proteins inside living cells

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Herein, we present a simple readout of the binding between a chemical drug and its target proteins in the cytoplasm by using a two-step bioorthogonal labeling method combined with spatially-localized expression of proteins. Dasatinib was modified with transcyclooctene (TCO), and its cytoplasmic target kinases were expressed in intracellular compartments, such as endosomes and F-actins. After bioorthogonal labeling, the colocalization between Dasatinib and its target proteins was observed in intracellular compartments
Publisher
ROYAL SOC CHEMISTRY
Issue Date
2016-07
Language
English
Article Type
Article
Keywords

LIVE CELLS; TRYPANOSOMA-BRUCEI; FAMILY KINASES; CHEMISTRY; PROBES; DRUG; INHIBITION; IMATINIB; REVEAL; DESIGN

Citation

CHEMICAL COMMUNICATIONS, v.52, no.79, pp.11764 - 11767

ISSN
1359-7345
DOI
10.1039/c6cc07011f
URI
http://hdl.handle.net/10203/213916
Appears in Collection
CH-Journal Papers(저널논문)
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