Exosome engineering for efficient intracellular delivery of soluble proteins using optically reversible protein-protein interaction module

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Nanoparticle-mediated delivery of functional macromolecules is a promising method for treating a variety of human diseases. Among nanoparticles, cell-derived exosomes have recently been highlighted as a new therapeutic strategy for the in vivo delivery of nucleotides and chemical drugs. Here we describe a new tool for intracellular delivery of target proteins, named 'exosomes for protein loading via optically reversible protein-protein interactions' (EXPLORs). By integrating a reversible protein-protein interaction module controlled by blue light with the endogenous process of exosome biogenesis, we are able to successfully load cargo proteins into newly generated exosomes. Treatment with protein-loaded EXPLORs is shown to significantly increase intracellular levels of cargo proteins and their function in recipient cells in vitro and in vivo. These results clearly indicate the potential of EXPLORs as a mechanism for the efficient intracellular transfer of protein-based therapeutics into recipient cells and tissues
Publisher
NATURE PUBLISHING GROUP
Issue Date
2016-07
Language
English
Article Type
Article
Citation

NATURE COMMUNICATIONS, v.7

ISSN
2041-1723
DOI
10.1038/ncomms12277
URI
http://hdl.handle.net/10203/212917
Appears in Collection
BiS-Journal Papers(저널논문)BS-Journal Papers(저널논문)
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