The magnitude and temporal variations of wind speed considerably influence aerodynamic and structural responses of MW-sized horizontal axis wind turbines. Thus, this paper investigates the variations in airloads and blade behavior of a wind turbine blade resulting from operations in sheared and turbulent flow conditions. First, in order to validate the present methods, comparisons of aerodynamic results were made among the blade element momentum method, free-wake method, and numerical results from the previous studies. Then, the validated methods were applied to a national renewable energy laboratory 5 MW reference wind turbine model for fluid-structure interaction analyses. From the numerical simulations, it can be clearly seen that unfavorable airloads and blade deformations occur due to the sheared and turbulent flow conditions. In addition, it is clear that wake impacts are not as substantial at those of high wind speeds; however, the effects obviously affect the aerodynamic and structural behaviors of the blade at lower wind speeds. Therefore, it is concluded that the numerical results markedly indicate the demand for accurate assessment of wake dynamics for accurate estimations of the aerodynamic and structural responses for sheared and turbulent flow environments.