Characterization and evaluation of corn steep liquid in acetone-butanol-ethanol production by Clostridium acetobutylicum

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Corn steep liquor (CSL) obtained from a commercial starch manufacturing process was analyzed and tested as a complex nutrient source for ABE (acetone, butanol, and ethanol) production by Clostridium acetobutylicum PJC4BK_AdhE1. CSL contained a wealth of trace elements and nitrogenous components, proteins and amino acids. ABE production increased the content of CSL was raised up to 6% (v/v) in medium and then decreased at higher contents. In 6% CSL-containing medium, C. acetobutylicum PJC4BK_AdhE1 produced 21.4 g/L of ABE with a yield of 0.41 g/g in 18 h of fermentation. Although the final titer of ABE was similar in CSL containing media and Clostridial Growth Medium (CGM, containing yeast extract and asparagines as complex nutrients), the yield and productivity of ABE in 6% CSL-containing medium were found to be higher than 32 and 26%, respectively.
Publisher
KOREAN SOC BIOTECHNOLOGY & BIOENGINEERING
Issue Date
2013-04
Language
English
Article Type
Article
Keywords

BEIJERINCKII BA101; ATCC 824; FERMENTATION; SOLVENTOGENESIS; INACTIVATION; BUTYRATE; WATER

Citation

BIOTECHNOLOGY AND BIOPROCESS ENGINEERING, v.18, no.2, pp.266 - 271

ISSN
1226-8372
DOI
10.1007/s12257-012-0619-8
URI
http://hdl.handle.net/10203/174064
Appears in Collection
CBE-Journal Papers(저널논문)
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