Heterologous Expression of a Putative K+/H+ Antiporter of S. coelicolor A3(2) Enhances K+, Acidic-pH Shock Tolerances, and Geldanamycin Secretion

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Heterologous expression of a putative K+/H+ antiporter of Streptomyces coelicolor A3(2) (designated as sha4) in E. coli and Streptomyces hygroscopicus JCM4427 showed enhanced tolerance to K+ stress, acidic-pH shock, and/or geldanamycin production under K+ stress. In a series of K+ extrusion experiments with sha4-carrying E. coli deficient in the K+/H+ antiporter, a restoration of impaired K+ extrusion activity was observed. Based on this, it was concluded that sha4 was a true K+/H+ antiporter. In different sets of experiments, the sha4-carrying E. coil showed significantly improved tolerances to K+ stresses and acidic-pH shock, whereas sha4-carrying S. hygroscopicus showed an improvement in K+ stress tolerance only. The sha4-carrying S. hygroscopicus showed much higher geldanamycin productivity than the control under K+ stress condition. In another set of experiments with a production medium, the secretion of geldanamycin was also significantly enhanced by the expression of sha4.
Publisher
KOREAN SOC MICROBIOLOGY & BIOTECHNOLOGY
Issue Date
2013-02
Language
English
Article Type
Article
Keywords

NA+/H+ ANTIPORTER; ESCHERICHIA-COLI; BACILLUS-SUBTILIS; SALT-STRESS; HELICOBACTER-PYLORI; STREPTOMYCES; OVEREXPRESSION; ARABIDOPSIS; H+; TRANSPORTERS

Citation

JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY, v.23, no.2, pp.149 - 155

ISSN
1017-7825
DOI
10.4014/jmb.1207.07030
URI
http://hdl.handle.net/10203/173462
Appears in Collection
CBE-Journal Papers(저널논문)
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