Dual functional, polymeric self-assembled monolayers as a facile platform for construction of patterns of biomolecules

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We report a facile approach to the construction of patterns of biomolecules based on polymeric self-assembled monolayers (pSAMs) that possess dual functions: "bio-reactive (post-functionalizable)" and "bio-inert (anti -biofouling)" properties. To prepare pSAMs on Si/SiO2 wafers were synthesized new random copolymers by radical polymerization of poly(ethylene glycol) methyl ether methacrylate (PEGMA), 3-(trimethoxysilyl)propyl methacrylate (TMSMA), and N-acryloxysuccinimide (NAS), and referred to as poly(TMSMA-r-PEGA-r-NAS).Poly(TMSMA-r-PEGMA-r-NAS) was designed to play triple roles: "surface-reactive", "bio-reactive", and "bio-inert" ones. The pSAMs of poly (TMSMA-r-PEGMA-r-NAS) were formed on Si/SiO2 wafers with 1h incubation of the substrates in the polymer solution, which showed approximately a 1 nm-thick film as measured by ellipsometry. After the formation of the pSAMs. the feasibility of the pSAMs as a dual functional surface (bio-inert and bio-reactive properties) was examined. The ability of the pSAMs to block nonspecific adsorption of proteins was evaluated against bovine serum albumin as a model protein. High-resolution N(1s) X-ray photoelectron spectroscopy (XPS) analysis on the protein adsorption revealed that significant reduction up to similar to 97% was observed compared to the unmodified Si/SiO2 wafer. In addition, micropatterns of streptavidin with high signal-to-noise ratios were achieved using microcontact printing (mu CP) of NH2-bearing biotin onto the pSAMs of poly(TMSMA-r-PEGMA-r-NAS) on glass slides, which suggests that other biomolecules could also be efficiently immobilized onto the pSAMs with high specificity while minimizing nonspecific adsorption. On the other hand, the surface density of both bio-reactive and anti-biofouling functionality could be tailored by simply changing initial feed ratios of each monomer in the polymer synthesis: different molar ratios of the bio-reactive group (NAS: 33%, 20%, and 10%, respectively) were employed. When micropatterns of streptavidin were constructed, the pSAMs with 33% NAS moiety showed the highest immobilization of the protein. Taken together, the present dual functional, random copolymers may have warrant applications in the field of biosensors and biochips.
Publisher
AMER CHEMICAL SOC
Issue Date
2007-10
Language
English
Article Type
Article
Keywords

PROTEIN MICROARRAYS; ENZYME MICROARRAYS; SOFT LITHOGRAPHY; HIGH-THROUGHPUT; ARRAYS; ORGANIZATION; MICROSCOPY; SURFACES; BINDING; CHIPS

Citation

LANGMUIR, v.23, no.22, pp.10902 - 10905

ISSN
0743-7463
DOI
10.1021/la7021903
URI
http://hdl.handle.net/10203/12153
Appears in Collection
CH-Journal Papers(저널논문)BS-Journal Papers(저널논문)
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