Functionalization of poly(oligo(ethylene glycol)methacrylate) films on gold and Si/SiO2 for immobilization of proteins and cells: SPR and QCM studies

Cited 130 time in webofscience Cited 0 time in scopus
  • Hit : 213
  • Download : 49
Thin films of a biocompatible and nonbiofouling poly(oligo(ethylene glycol) methacrylate) (pOEGMA) with various thicknesses were formed on gold and Si/SiO2 substrates by a combination of the formation of self-assembled monolayers (SAMs) terminating in bromoester-an initiator of atom transfer radical polymerization (ATRP)-and surface-initiated ATRP. After the formation of the pOEGMA films, terminal hydroxyl groups of side chains divergent from the methacrylate backbones were activated with N,N'-disuccinimidyl carbonate (DSC), and the DSC-activated pOEGMA films were reacted with (+)-biotinyl-3,6,9-trioxatmdecanediamine (Biotin-NH2) to form biotinylated pOEGMA films. By surface plasmon resonance experiments with the target protein (streptavidin) and model proteins (fibrinogen and lysozyme), we verified that the resulting films showed the enhanced signal-to-noise ratio (similar to 10-fold enhancement) for the biospecific binding of streptavidin compared with the biotinylated substrate prepared from carboxylic acid-terminated SAMs. Quartz crystal microbalance measurements were also carried out to obtain the surface coverage of streptavidin and fibrinogen adsorbed onto the biotinylated pOEGMA films with various thicknesses and to investigate the effect of film thicknesses on the biospecific binding of. streptavidin. Both the binding capacity of streptavidin and the signal-to-noise ratio of streptavidin/fibrinogen were found to be saturated at the 20 nm thick pOEGMA film. In addition, to demonstrate a wide applicability of the pOEGMA films, we constructed micropatterns of streptavidin and cells by microcontact-printing biotin-NH2 and poly-L-lysine onto the DSC-activated pOEGMA films, respectively.
Publisher
AMER CHEMICAL SOC
Issue Date
2007-12
Language
English
Article Type
Article
Keywords

TRANSFER RADICAL POLYMERIZATION; SELF-ASSEMBLED MONOLAYERS; SURFACE-PLASMON RESONANCE; RESISTANT SURFACES; BRUSHES; ADSORPTION; POLYMERS; COATINGS; SILICON; SENSORS

Citation

BIOMACROMOLECULES, v.8, no.12, pp.3922 - 3929

ISSN
1525-7797
DOI
10.1021/bm7009043
URI
http://hdl.handle.net/10203/12001
Appears in Collection
CH-Journal Papers(저널논문)
Files in This Item
This item is cited by other documents in WoS
⊙ Detail Information in WoSⓡ Click to see webofscience_button
⊙ Cited 130 items in WoS Click to see citing articles in records_button

qr_code

  • mendeley

    citeulike


rss_1.0 rss_2.0 atom_1.0