Tissue-engineered cartilage on biodegradable macroporous scaffolds: Cell shape and phenotypic expression

Cited 20 time in webofscience Cited 0 time in scopus
  • Hit : 374
  • Download : 55
Objective. The purpose of the study was to establish in vitro culture of chondrocytes on biodegradable, poly(D,L,lactic-co-glycolic acid) [PLGA] scaffolds. Study Design: Laboratory experiment using cartilage of rat rib and biodegradable scaffolds. Methods. Chondrocytes were cultured on a poly-hydroxyethyl methacrylate (poly-HEMA)-coated dish, proliferated, and transferred into the PLGA scaffolds. Phenotypic expression of cells was examined according to the condition of poly-HEMA coating. Morphological, biochemical, and immunohistochemical characteristics of cells cultured within PLGA scaffolds were also examined. Results: Chondrocytes cultured on a poly-HEMA-coated dish aggregated into distinct nodules containing large clusters of spherical cells and showed cartilage-specific phenotype, collagen type H. The results of immunostaining and reverse transcriptase-polymerase chain reaction of cells cultured within PLGA scaffolds showed cartilage-specific morphological appearance and structural characteristics such as lacunae and expression of collagen type H. Conclusion: The chondrocytes cultured on a poly-HEMA-coated dish and PLGA scaffolds showed chondrocyte-specific phenotypes and morphological appearance.
Publisher
Wiley-Blackwell
Issue Date
2002-09
Language
English
Article Type
Article
Keywords

ARTICULAR-CARTILAGE; POLYMER SCAFFOLDS; CHONDROCYTES; COLLAGEN; AGAROSE; DEFECTS; GELS

Citation

LARYNGOSCOPE, v.112, no.6, pp.1050 - 1055

ISSN
0023-852X
URI
http://hdl.handle.net/10203/11050
Appears in Collection
BS-Journal Papers(저널논문)
Files in This Item
This item is cited by other documents in WoS
⊙ Detail Information in WoSⓡ Click to see webofscience_button
⊙ Cited 20 items in WoS Click to see citing articles in records_button

qr_code

  • mendeley

    citeulike


rss_1.0 rss_2.0 atom_1.0