Probing the functional motifs of Escherichia coli 5S rRNA in relation to 16S rRNA using a SELEX experiment

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The function of 5S rRNA, a constituent of a large subunit of ribosome, is not dearly known yet. To identify RNA motifs interacting with 5S rRNA, and thereby to get an insight into the function of 5S rRNA in the ribosome, a SELEX (Systematic Evolution of Ligands by Exponential Enrichment) experiment was performed. RNA molecules binding to Escherichia coli 5S rRNA were selected from a 48-mer random sequence library through 12 rounds of selection, cloned, and sequenced. Two groups of the selected RNA molecules had the consensus sequences GCGG and GUGAAA, respectively, which are present in the segment, G(688) through A(696), of E. coli 16S rRNA. The gel mobility shift assay showed that 5S rRNA interacted with the 16S rRNA fragment containing the GCGG and GUGAAA sequences. The enzymatic protection experiment shows that the A(29)CCUGA(34) and G(51)AAGUG(56) sequences of 5S rRNA and the C(680)AGG(683) and G(688)CGG(691) sequences of the 16S rRNA fragment are involved in the interaction between the two RNA molecules. On the basis of this observation, we suggest that 5S rRNA and 16S rRNA play a role for the association of two ribosomal subunits.
Publisher
KOREAN CHEMICAL SOC
Issue Date
1999-11
Language
English
Article Type
Article
Keywords

IN-VITRO SELECTION; 23S RIBOSOMAL-RNA; BINDING RNA; L-ARGININE; LIGANDS; RECOGNITION; TRANSLATION; ANTIBIOTICS; MOLECULES; EVOLUTION

Citation

BULLETIN OF THE KOREAN CHEMICAL SOCIETY, v.20, no.11, pp.1335 - 1339

ISSN
0253-2964
URI
http://hdl.handle.net/10203/10631
Appears in Collection
CH-Journal Papers(저널논문)
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