DC Field | Value | Language |
---|---|---|
dc.contributor.author | Joo, Hyung-Joon | ko |
dc.contributor.author | Choi, Dong-Kyu | ko |
dc.contributor.author | Lim, Joon-Seo | ko |
dc.contributor.author | Park, Jin-Sung | ko |
dc.contributor.author | Lee, Seung-Hun | ko |
dc.contributor.author | Song, Suk-Hyun | ko |
dc.contributor.author | Shin, Jennifer Hyunjong | ko |
dc.contributor.author | Lim, Do-Sun | ko |
dc.contributor.author | Kim, In-June | ko |
dc.contributor.author | Hwang, Ki-Chul | ko |
dc.contributor.author | Koh, Gou-Young | ko |
dc.date.accessioned | 2013-03-12T12:17:12Z | - |
dc.date.available | 2013-03-12T12:17:12Z | - |
dc.date.created | 2013-02-14 | - |
dc.date.created | 2013-02-14 | - |
dc.date.issued | 2012-09 | - |
dc.identifier.citation | BLOOD, v.120, no.13, pp.2733 - 2744 | - |
dc.identifier.issn | 0006-4971 | - |
dc.identifier.uri | http://hdl.handle.net/10203/102289 | - |
dc.description.abstract | Successful differentiation and expansion of endothelial cells (ECs) from embryonic stem cell (ESC)-derived Flk1(+) mesodermal precursor cells (MPCs) requires supplementation of vascular endothelial growth factor-A (VEGF-A). While analyzing VEGF-A/VEGFR2 downstream signaling pathway that underlies the VEGF-A-induced differentiation and expansion of ECs, we fortuitously found that Rho-associated protein kinase (ROCK) inhibitor Y27632 profoundly promoted the differentiation and expansion of ECs from Flk1(+) MPCs while reducing the differentiation and expansion of mural cells. The ROCK suppression-induced expansion of ECs appears to have resulted from promotion of proliferation of ECs via activation of PI3-kinase-Akt signaling. The ECs obtained by the combination of ROCK suppression and VEGF-A supplementation faithfully expressed most pan-EC surface makers, and phenotypic analyses revealed that they were differentiated toward arterial EC. Further incubation of the ICAM2(+) ECs with Y27632 and VEGF-A for 2 days promoted expansion of ECs by 6.5-fold compared with those incubated with only VEGF-A. Importantly, the ROCK suppression- induced ECs displayed neovasculogenic abilities in vitro and in vivo. Thus, supplementation of ROCK inhibitor Y27632 along with VEGF-A in 2D Matrigel culture system provides a simple, efficient, and versatile method for obtaining ample amount of ESC-derived ECs at high purity suitable for use in therapeutic neovascularization. (Blood. 2012;120(13):2733-2744) | - |
dc.language | English | - |
dc.publisher | AMER SOC HEMATOLOGY | - |
dc.subject | VASCULAR PROGENITORS | - |
dc.subject | IN-VITRO | - |
dc.subject | SPROUTING ANGIOGENESIS | - |
dc.subject | MICROFLUIDIC PLATFORM | - |
dc.subject | VEGF | - |
dc.subject | PATHWAY | - |
dc.subject | VASCULOGENESIS | - |
dc.subject | ARTERIAL | - |
dc.subject | KINASES | - |
dc.subject | PROTEIN | - |
dc.title | ROCK suppression promotes differentiation and expansion of endothelial cells from embryonic stem cell-derived Flk1(+) mesodermal precursor cells | - |
dc.type | Article | - |
dc.identifier.wosid | 000311615800029 | - |
dc.identifier.scopusid | 2-s2.0-84866865798 | - |
dc.type.rims | ART | - |
dc.citation.volume | 120 | - |
dc.citation.issue | 13 | - |
dc.citation.beginningpage | 2733 | - |
dc.citation.endingpage | 2744 | - |
dc.citation.publicationname | BLOOD | - |
dc.identifier.doi | 10.1182/blood-2012-04-421610 | - |
dc.contributor.localauthor | Shin, Jennifer Hyunjong | - |
dc.contributor.localauthor | Kim, In-June | - |
dc.contributor.localauthor | Koh, Gou-Young | - |
dc.contributor.nonIdAuthor | Lim, Do-Sun | - |
dc.contributor.nonIdAuthor | Hwang, Ki-Chul | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordPlus | VASCULAR PROGENITORS | - |
dc.subject.keywordPlus | IN-VITRO | - |
dc.subject.keywordPlus | SPROUTING ANGIOGENESIS | - |
dc.subject.keywordPlus | MICROFLUIDIC PLATFORM | - |
dc.subject.keywordPlus | VEGF | - |
dc.subject.keywordPlus | PATHWAY | - |
dc.subject.keywordPlus | VASCULOGENESIS | - |
dc.subject.keywordPlus | ARTERIAL | - |
dc.subject.keywordPlus | KINASES | - |
dc.subject.keywordPlus | PROTEIN | - |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.